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  • Investigating the effect of the extracted polysaccharide from Brittle star (Ophiocoma erinaceus) on Immunological parameters in the experimental model of liver cirrhosis

  • Mohammadreza Pourmohammad,1 Jian Khayatzadeh,2,* Khadijeh Afshoun,3 Shima Afshari,4 Javad Baharara,5
    1. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
    2. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
    3. Clinical Research Development Unit, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
    4. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
    5. Department of Biology and Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran


  • Introduction: Hepatoprotective potential of marine natural products such as polysaccharides represented therapeutic potential in hepatological researches. In this study, total polysaccharide from brittle star [Ophiocoma erinaceus (O.erinaceus)] was extracted, immunotherapy efficacy of Persian Gulf brittle star polysaccharide was investigated in carbon tetrachloride-induced acute liver injury in rats
  • Methods: To extract polysaccharide, dried brittle stars were ground and extracted mechanically. Western blot analysis was conducted to assess the expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and adenosine A2A receptor. The rats were fed with 0.1%CCl4 (0.2 mL/10 g) mixed with soybean oil for 7 days. The rats in the BSP treatment groups administered intragastrically with Brittle Star Extracted Polysaccharide at the level of 12.5, 25, 37.5 mg/kg body weight (b.w.)/day for7days after CCl4 induction.
  • Results: Carbon tetrachloride significantly decreased the rats’ body weight, but it increased their livers weight. The polysaccharide detection methods demonstrated isolation of crude polysaccharide from Persian Gulf brittle star. TNF-α, IL-1β, and IL-6 expressions were decreased by Brittle Star Extracted Polysaccharide treatment in CCl4-intoxicated rats. Also BSP administration overexpressed adenosine A2A receptor in CCl4-intoxicated rats. Histopathological evaluations showed extensive liver damage. On the other hand, treatment with extracted polysaccharide from Brittle star increased liver weight, reduced body weight and significantly altered other induced changes by carbon tetrachloride on liver structure such as hepatocytes number, Kupffer cells, and arteritis, which indicated the improvement of damaged liver tissue.
  • Conclusion: The therapeutic efficacy of Brittle Star Extracted Polysaccharide also can be expected for CCl4-intoxicated rats. The current research suggests that Brittle Star Extracted Polysaccharide may be used for the therapeutic agent of CCl4-intoxicated rats. Therefore, these findings proposed new insight into Hepatoprotective potential of brittle star polysaccharide as a promising agent in hepatic treatment.
  • Keywords: Carbon tetrachloride, Brittle star, Proinflammatory cytokines, Polysaccharides

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