INTERNATIONAL CONGRESS OF
PharmacoGenetics
The effect of vanillic acid on the expression of osteocalcin and osteopentin genes in rat bone marrow mesenchymal stem cells
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Mohammadreza Pourmohammad,1 Khadijeh Afshoun,2 Jina Khayatzadeh,3,* Javad Baharara,4 Saeedeh Zafar Balanejad,5
1. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
2. Clinical Research Development Unit, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
3. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
4. Department of Biology and Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
5. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
- Introduction: Bone tissue engineering is a progressive approach in bone tissue repair in medical science. Induction of osteogenic differentiation is a very important process for bone repair. The aim of this study was to investigate the effect of vanillic acid on the expression level of osteocalcin and osteopentin genes in the differentiation of rat bone marrow mesenchymal stem cells.
- Methods: For this purpose, mesenchymal stem cells were isolated from the bone marrow of Wistar rats and identified with specific antibodies against surface markers CD45, CD44 and CD31. Mesenchymal stem cells of rat bone marrow were treated with different concentrations of vanillic acid. Cytotoxicity was evaluated by MTT method after 48 hours. The differentiation effects of vanillic acid on stem cells to osteoblasts were investigated by alizarin red staining methods and Real Time-PCR for specific markers of osteoblasts, osteocalcin and osteopentin.
- Results: The results of the treatment of the cells showed that vanillic acid had no significant effect on the viability of these cells up to a concentration of less than 0.5 μM; The concentration that led to the death of 50% of the cells was equal to 10 μM, and higher concentrations led to the death of cells in the first hours of the treatment, therefore, the concentrations of 0.5, 1 and 2 μM were chosen to investigate the advancement of cell differentiation. Alizarin staining showed rejection. Vanillic acid led to bone differentiation on day 21 in a dose-dependent manner. The results of Real Time-PCR showed that the gene expression of osteocalcin and osteopentin increased significantly in the treatment groups.
- Conclusion: The results of this study showed that vanillic acid increases osteogenic differentiation in mesenchymal stem cells derived from the bone marrow of Wistar rats.
- Keywords: vanillic acid, rat, osteopentin, mesenchymal stem cells, osteocalcin
