INTERNATIONAL CONGRESS OF
PharmacoGenetics
Investigating the effect of the extracted polysaccharide from Brittle star (Ophiocoma erinaceus) on biochemical parameters in the experimental model of liver cirrhosis
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Mohammadreza Pourmohammad,1 Jian Khayatzadeh,2,* Khadijeh Afshoun,3 Shima Afshari,4 Javad Baharara,5
1. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
2. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
3. Clinical Research Development Unit, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
4. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
5. Department of Biology and Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
- Introduction: Brittle star possess bioactive compounds which confer the wound healing capacity and regenerative potency of damaged arms and organisms to this creature. The purpose of this study was to investigate the protective effect of extracted polysaccharide from Brittle star on biochemical parameters in the experimental model of liver damage caused by carbon tetrachloride in adult male Wistar rats.
- Methods: To extract polysaccharide, dried brittle stars were ground and extracted mechanically. We analyzed the levels of GSH, TBARS, H2O2, SOD, CAT, GPx, GST, LOX markers. The rats were fed with 0.1%CCl4 (0.2 mL/10 g) mixed with soybean oil for 7 days. The rats in the BSP treatment groups administered intragastrically with Brittle Star Extracted Polysaccharide at the level of 12.5, 25, 37.5 mg/kg body weight (b.w.)/day for7days after CCl4 induction.
- Results: Carbon tetrachloride significantly decreased the rats’ body weight, but it increased their livers weight. Histopathological evaluations showed .extensive liver damage. On the other hand, treatment with extracted polysaccharide from Brittle star increased liver weight, reduced body weight and significantly altered other induced changes by carbon tetrachloride on liver structure such as hepatocytes number, Kupffer cells, and arteritis, which indicated the improvement of damaged liver tissue. Brittle Star Extracted Polysaccharide treated rats significantly increased SOD, CAT, GPx, GST activities and GSH level.
- Conclusion: It was found that extracted polysaccharide from Brittle star can exert protective effects on liver damages induced by carbon tetrachloride on male Wistar rat.
- Keywords: Carbon tetrachloride, Brittle star, Polysaccharides, Antioxidants, Stress oxidative
